Coding

Part:BBa_I728000:Design

Designed by: Forrest Liau   Group: iGEM07_MIT   (2007-05-26)


Pseudomonas fluorescens enzyme for vanilla scent synthesis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 643
    Illegal PstI site found at 1618
    Illegal PstI site found at 1690
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 643
    Illegal PstI site found at 1618
    Illegal PstI site found at 1690
    Illegal NotI site found at 1727
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 643
    Illegal PstI site found at 1618
    Illegal PstI site found at 1690
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 643
    Illegal PstI site found at 1618
    Illegal PstI site found at 1690
    Illegal NgoMIV site found at 294
    Illegal NgoMIV site found at 1107
    Illegal NgoMIV site found at 1475
    Illegal NgoMIV site found at 1629
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 667


Design Notes

Change stop codon to TAATAA.


Source

GenBank Accession number: AJ536325

References